Research progress in the treatment of liver cancer with disulfiram / 中华肝胆外科杂志

Disulfiram, a drug that has been used for alcohol dependence. As an approved drug in clinical medicine, disulfiram can be used as the anticancer drug in the treatment of breast cancer, prostate cancer, glioblastoma, lung cancer, etc. This paper summarized the mechanism of disulfiram for anticancer treatment and the function for liver cancer therapy, and we also analyzed the potential mechanism of disulfiram for the treatment of liver cancer and its’ value in the clinical application.

Mandibular angle osteotomy combined with chin augmentation with high-density polyethylene for one stage lower facial recontouring / 中华医学美学美容杂志

Objective:To investigate the feasibility of mandibular angle osteotomy combined with high-density polyethylene (MEDPOR) prosthesis implantation for one-time lower facial contouring of the protuberance and related surgical precautions.Methods:A total of 18 patients who were treated in the oral and maxillofacial surgery of Yangzhou First People＇s Hospital from June 2010 to August 2016 were selected to complete the mandibular angle osteotomy combined with MEDPOR prosthesis implantation in the lower part of the prosthetic plane. The patients were followed up regularly and the effect of surgery was observed.Results:All 18 patients were operated successfully with an average time of 2 hours. The effect of operation was satisfactory. Except for one patient whose hematoma caused local bruise to subside completely in 2 weeks, all the others were discharged smoothly in one week after operation. After 2 months to 3 years of follow-up, the effect was satisfactory.Conclusions:Mandibular angle osteotomy combined with MEDPOR prosthesis is safe and effective.

Effect of delayed cord clamping on cerebral tissue oxygen saturation in newborns / 中华实用儿科临床杂志

Objective@#To investigate the influence of different delayed cord clamping(DCC) time on cerebral tissue oxygen saturation in normal newborns after birth and to explore the causes.@*Methods@#From November 2018 to February 2019, 164 healthy full term newborns were delivered in the Department of Obstetrics, Binhu Hospital, the Southern District of the Third Affiliated Hospital of Anhui Medical University.The newborns were divided into early cord clamping (ECC) group, DCC groups for 1 minute, 2 minutes and 3 minutes according to the different cord clamping time, and the changes of cerebral tissue oxygen saturation index (cTOI) and cerebral tissue hemoglobin index(cTHI) were monitored by near infrared spectroscopy (NIRS) for 15 minutes after birth.@*Results@#After birth, cTOI increased gradually but cTHI was basically stable.The cTOI in DCC group was higher than ECC group, but there was no difference between group 3 and group 4.In ECC group, the cTOI tended to be stable about 6 minutes after birth, and the stable range was 55%-59%.In DCC group, group 2 tended to be stable about 8 minutes after birth, and the stable range was 59%-64%.Group 3 and group 4 tended to be stable about 10 minutes after birth, the stable range was 64%-69%.There was no difference in cTHI among the groups.@*Conclusions@#Delayed cord clamping can increase cerebral oxygenation in newborns.The cause may not be due to the direct increase in cerebral blood flow, but the increase in brain oxygen content, and the optimal duration of DCC is 2 minutes.

Effect of delayed cord clamping on cerebral tissue oxygen saturation in newborns / 中华实用儿科临床杂志

Objective To investigate the influence of different delayed cord clamping(DCC) time on cerebral tissue oxygen saturation in normal newborns after birth and to explore the causes.Methods From November 2018 to February 2019,164 healthy full term newborns were delivered in the Department of Obstetrics,Binhu Hospital,the Southern District of the Third Affiliated Hospital of Anhui Medical University.The newborns were divided into early cord clamping (ECC) group,DCC groups for 1 minute,2 minutes and 3 minutes according to the different cord clamping time,and the changes of cerebral tissue oxygen saturation index (cTOI) and cerebral tissue hemoglobin index (cTHI) were monitored by near infrared spectroscopy (NIRS) for 15 minutes after birth.Results After birth,cTOI increased gradually but cTHI was basically stable.The cTOI in DCC group was higher than ECC group,but there was no difference between group 3 and group 4.In ECC group,the cTOI tended to be stable about 6 minutes after birth,and the stable range was 55％-59％.In DCC group,group 2 tended to be stable about 8 minutes after birth,and the stable range was 59％-64％.Group 3 and group 4 tended to be stable about 10 minutes after birth,the stable range was 64％-69％.There was no difference in cTHI among the groups.Conclusions Delayed cord clamping can increase cerebral oxygenation in newborns.The cause may not be due to the direct increase in cerebral blood flow,but the increase in brain oxygen content,and the optimal duration of DCC is 2 minutes.

Topical ozone therapy: An innovative solution to patients with herpes zoster / 中南大学学报(医学版)

Objective:To observe the clinical efficacy and safety of topical ozone therapy for patients with herpes zoster by reflectance confocal microscopy (RCM).Methods:A total of 60 patients with herpes zoster were divided into a control group and an ozone treatment group (n=30).In the control group,patients took oral valacyclovir tablets or granules (0.3 g per day,three times a day) and they were subjected to local weak laser irradiation treatment plustopical 2％ mupirocin ointment twice a day.In the ozone group,the treatment is same as the control group except mupirocin ointment was replaced with topical ozone treatment (hydrotherapy every day plus ozonated oil twice a day).The clinical symptoms,discoid cell and adverse reactions were observed and taken records at day 0,3,7 and 14.Statistical analysis was performed to compare the clinical efficacy between the 2 groups.Results:On the seventh day of treatment,the discoid cells of the ozone group disappeared,and the difference between the control group and the ozone group was statistically significant (P＜0.05).The difference of decreased percentage of pain scores at each time point between the 2 groups was statistically significant (P＜0.05).The clinical efficacy was 100％ in the ozone group and 86.7％ in the control group,with significant difference between the 2 groups (P＜0.05).Conclusion:Topical ozone therapy in patients with herpes zoster is helpful in relieving pain,shortening the course as well as improving the clinical efficacy without obvious adverse reactions.It is worth to be popularized.

Topical ozone application: An innovative therapy for infantile atopic dermatitis / 中南大学学报(医学版)

Objective:To evaluate the clinical efficacy and safety of the innovative topical ozone therapy for infantile atopic dermatitis.Methods:Sixty children with atopic dermatitis were divided into a treatment group and a control group.The treatment group was showered with ozonated water (3-5 times a week) and smeared with ozonated oil (twice a day),while the control group was washed with warm running water and smeared with base oil,adding moisturizer if necessary.The treatment course was 2 weeks.Efficacy and side effect were evaluated.Results:The skin exudation was reduced and erosion was healing after 3-5 days topical ozone therapy for infantile atopic dermatitis.The effective rates were 80.0％ and 20.0％ in the treatment group and control group for 1 week,and 89.6％ and 30.7％ for 2 weeks,respectively,with significant difference between the 2 groups (P＜0.001).Conclusion:Innovative treatment of infantile atopic dermatitis with topical ozone application is safe and effective,which is worth popularizing in clinic.

Effect of ozone on Staphylococcus aureus colonization in patients with atopic dermatitis / 中南大学学报(医学版)

Objective:To verify the effect of ozone on Staphylococcus aureus (S.aureus) colonization in patients with atopic dermatitis (AD) and its correlation with the patient's status.Methods:A total of 12 patients with moderate or severe AD,aged from 6 to 65 years,were recruited from outpatient of the Third Xiangya Hospital.The treatment sides were showered with ozonated water and smeared with ozonated oil for 7 days (twice a day),while the control sides were washed with warm running water and smeared with base oil.At different time points,the severity scoring ofatopic dermatitis (SCORAD) scores,sleep and pruritus scores were assessed and compared between the two sides.Meanwhile,plate cultivation was used to quantitatively detect the changes ofS.aureus colonization in skin lesions.Results:After 7 days treatment,erythema and pimples were decreased in the treatment sides.The clear skin texture,smooth skin,improved skin lesions were also observed by dermoscopic examination.The results of reflectance confocal microscopy (RCM) demonstrated that the parakeratosis was improved,the structures were clearer,and the inflammatory cells infiltration was reduced after ozone treatment for 7 days.After ozone treatment for 3 and 7 days,the S.aureus colonization in the treatment sides decreased by (75.55±21.81)％ and (97.24±2.64)％ respectively.Compared to that of control sides,the percentage of S.aureus colony after ozone treatment for 7 days decreased significantly (P＜0.01).After ozone treatment for 7 days,the SCORAD scores,sleep and pruritus scores were significantly decreased (all P＜0.01).There was a linear correlation between the decreasing percentage of S.aureus colony and the declining percentage of SCORAD scores in AD patients.Conclusion:Topical ozone therapy can effectively reduce S.aureus colony in skin lesions and alleviate the severity of AD patients with moderate to severe degree.

Efficacy of combination of ozonated water with oil for treatment of tinea pedis / 中南大学学报(医学版)

Objective:To evaluate efficacy of combined therapy with ozonated water and oil on patients with tinea pedis.Methods:A total of 60 patients with tinea pedis were divided into 2 groups in a randomized and blinded test.Patients in a control group were treated with naftinfine hydrochloride and ketoconazole cream once a day.Patients in an ozone group were treated with ozonated water bath and then ozonated oil topical application once a day.Patients in the 2 groups were treated for 4 weeks.Clinical and laboratory data were collected for both groups at the end of the 1st week,the 2nd week,and the 4th week.The Pearson chi-square was performed to compare scores of the clinical signs and symptoms (CSS) and the mycological result between the 2 groups.Independent samples T-test was performed to compare the curative effect between the 2 groups.Results:After 4 weeks' treatment,6 patients were positive in the control group determined by mycological examination while 1 patient was positive in the ozone group,with no significant difference between the 2 groups (P＞0.05).Changes in CSS at the end of the 1st week,2nd week,and 4th week were obtained and showed no significant difference between the 2 groups at the 3 different time points (P＞0.05).No side effects were observed.Conclusion:Combination of ozonated water with oil is effective on treatment oftinea pedis and it shows no side effects.

Attenuation rules and germicidal efficacy of ozoneted water / 中南大学学报(医学版)

Objective:To determine initial concentrations of ozonated water under different temperatures,attenuation rules ofozonated water under the room temperature (25 ℃),and to inspect the effects ofozonated water under different concentrations on common microorganisms.Methods:The online test method and the plate cultivation method were employed to check the concentrations and killing rates on common microorganisms of ozonated water produced by HZ-2601 B Ozone Water Generating Instrument.Results:The initial concentrations of ozonated water at 20,25,30,35,and 40 ℃ were 4.38,4.26,3.12,2.76,and 1.31 mg/L,respectively.The ozonated water was rapidly attenuated at first 10 min.The concentration ofozonated water still remained at 1.06 mg/L and 0.37 mg/L at 25 and 30 ℃ after 30 min.The average killing rates for Pseudornonas aeruginosa,Escherichia coli,Staphylococcus aureus,methicillin-resistant Staphylococcus aureus,and Candida albicans in 1.0 mg/L ozonated water for 1 min were 99％,100％,100％,100％,and 100％,respectively.The average killing rates of Escherichia coli,Staphylococcus aureus,methicillin-resistant Staphylococcus aureus,Pseudomonas aeruginosa,and Candida albicans in 0.3 mg/L ozonated water for 1 min were 100％,100％,100％,95％,and 92％,respectively.Conclusion:The initial concentrations of ozonated water produced by HZ-2601 B Ozone Water Generating Instrument decrease with the increase of temperature.Ozonated water under 20-30 ℃ has good sterilization effect on common microorganisms.

Safety evaluation for medical ozone oil on skin / 中南大学学报(医学版)

Objective:To evaluate skin irritation,acute toxicity,and allergy of medical ozone oil for clinical application.Methods:In contrast to their left and right side irritation,one or more skin irritation tests were performed on the intact and damaged skins of guinea pigs.With the maximum concentration,acute skin toxicity test was applied on the intact and damaged skins of rats.Active cutaneous anaphylaxis was applied to the guinea pigs.Results:High concentration (ozone consumption:150 g/L) of medical ozone oil showed a slight irritation on the broken skin of guinea pigs,while low concentrations did not show skin irritation.Medical ozone oil had no obvious acute toxicity to rats.The medical ozone oil and base oil showed mildallergy for the skin of guinea pig.Conclusion:The irritation of medical ozone oil is related to its concentration.With appropriateconcentration and duration of treatment,medical ozone oil is safe.

Serum interleukin-31 level and pruritus in atopic dermatitis: A Meta-analysis / 中南大学学报(医学版)

Objective:To investigate associations of interleukin-31 (IL-31) and pruritus in atopic dermatitis (AD) with Meta-analysis.Methods:Materials were extracted from the citations listed in the following databases:PubMed,Science Direct,Web of Science and Cochrane.Key search terms included:atopic dermatitis,pruritus,and IL-31.The Meta-analysis was used to analyze the correlation between pruritws in AD and IL-31 expression level.Results:The Meta-analysis showed that serum IL-31 levels were higher in AD patients than those in the healthy controls.The levels of IL-31 were higher in severe AD patients than those in the mild and moderate AD patients.Moreover,a positive correlation between serum IL-31 levels and severity of pruritus was identified.Conclusion:Increased serum levels of IL-31 generally exist in the AD patients,and it may accelerate the pruritus in the AD patients.

Correlation between onset frequencies and duration of symptoms and early stroke risk in patients with transient ischemic attack / 国际脑血管病杂志

Objective To investigate the correlation between onset frequencies and duration of symptoms and early stroke risk in patients with transient ischemic attack (TIA).Methods From January 2013 to July 2017,inpatients with TIA at the Department of Neurology,Lu'an People's Hospital were enrolled retrospectively.According to the findings of head CT or MRI reexaminations within 72 h after the first TIA,the patients were divided into non-stroke group and stroke group.The demographic and clinical data of both groups were compared.Results A total of 171 patients with TIA were enrolled,including 54 in the stroke group and 117 in the non-stroke group.There were significant differences in sex,atrial fibrillation,using anticoagulant drug before onset,baseline blood pressure (systolic and diastolic blood pressure),lowdensity lipoprotein cholesterol,high-density lipoprotein cholesterol,fasting blood glucose,TIA etiology (cardiogenic embolism),TIA frequency (≥2 times),duration of TIA symptoms,ABCD2 score (6 and 7),and using antihypertensive drugs after onset in both groups.Multivariable logistic regression analysis indicated that high diastolic blood pressure (odds ratio [OR] 1.121,95％ confidence interval [CI] 1.016-1.238;P =0.023),high fasting glucose (OR 10.866,95％ CI 3.658-30.945;P =0.001),ABCD2 score 6 (OR 4.221,95％ CI 1.906-9.352;P ＜ 0.001),ABCD2 score 7 (OR 4.207,95％ CI 2.040-9.961,P ＜ 0.001),duration of symptoms ≥ 60 min(OR 3.682,95 ％ CI 1.961-9.989;P ＜ 0.001),and frequency of TIA onset ≥2 (OR 4.220,95％ CI 1.953-9.118;P＜0.001) were the independent risk factors for early TIA,and the use of anticoagulants before onset was an independent protective factor (OR 0.300,95％ CI 0.097-0.923;P =0.036).Conclusion TIA patients with recurrent onset (≥2 times) and duration of symptoms longer than 60 min are more likely to develop ischemic stroke in a short term.

Influences of water supply sources of dental comprehensive treatment platform on intra-pipeline water quality / 医疗卫生装备

Objective To investigate the impacts of water supply on water quality in the pipeline of the dental comprehensive treatment platform to contribute to pollution control.Methods Totally 8 platforms from the stomatological department underwent 2-a detection and tracing.The water sources included tap water,distilled water and filtered water,and the discharge water went through sampling and bacteriological analysis before and after disinfection.Results The mean numbers of colonies by tap water,distilled water and filtered water were (472±385),(380±372) and (446±382) cfu/ml respectively,and the qualification rates by tap water,distilled water and filtered water were 33.3％,45.8％ and 37.5％ respectively.All the colonies numbers were limited within 0 and 60 cfu/ml with the qualification rate being 100％ after disinfection.Conclusion Water source cannot relieve bacterial infection effectively,and disinfection eliminates bacteria and improves water quality in the pipeline of the dental comprehensive treatment platform.

Epicutaneous sensitization with ovalbumin, staphylococcal enterotoxin B and vitamin D analogue induces atopic dermatitis in mice / 中南大学学报(医学版)

Objective:To illuminate a method for establishment of a cost-efficient atopic dermatitis (AD) mouse model by topical application of ovalbumin (OVA),super-antigen staphylococcal enterotoxin B (SEB),and calcipotriene ointment (CO) on the back of BALB/c mice.Methods:Experimental mice were topically treated with OVA/SEB or OVA/SEB/CO every other day during 15 days of induction.Clinical alterations on the skin area were monitored every other day.Epidermal thickness were measured by reflectance confocal microscope (RCM) before harvest.Inflammatory cells in skin biopsies were marked by hematoxylin-eosin (HE) staining.Blood sample and skin biopsies were measured by ELISA and quantitative real-time PCR to detect the expression of IL-2,IL-4,IL-31,interferon (IFN)-γ,tumor necrosis factor (TNF)-α pruritus-associated nerve growth factor (NGF),and serum IgE.Results:Human AD-like cutaneous local inflammatory reaction was characterized by the accumulation of inflammatory cells,increased epidermal thickness and serum IgE levels as well as Th1 cell-associated cytokines (IFN-γ,TNF-α),Th2 cell-associated cytokines (IL-4,IL-31),and NGF in the OVA/SEB/CO group compared with that in the normal control group or the OVA/ SEB group.Conclusion:OVA/SEB/CO can induce an AD-like mouse model with lower economic and time consumption.

Not Available / 中南大学学报(医学版)

OBJECTIVE@#To evaluate the efficacy and influential factors for 308 nm excimer laser in the treatment of stable vitiligo patients.
@*METHODS@#A total of 207 stable vitiligo patients with 1 763 patches were treated with 308 nm excimer laser. Open-label study was carried out to investigate the efficacy and safety regarding the treatment with 308 nm excimer laser, and to compare the response under different conditions including gender, age, duration, lesion location, and hair color.
@*RESULTS@#After treatment, 560 (31.8%) patches achieved 100% repigmentation, 650 (36.9%) lesions showed 75%-99% repigmentation, 189(10.7%) showed 50%-75% repigmentation, 231(13.1%) showed 25%-49% repigmentation, 108(6.1%) showed 1%-24% repigmentation, 25(1.4%) displayed no response. The rates of total excellent response (50%-100% repigmentation) in underage patients was 86.9%, much higher than that in adult patients (P<0.001). Total excellent response rates was 90.6% in disease duration <2 years, and 40.7% in disease duration ≥2 years. Lesions on the faciocervical region responded better than trunk and limbs, showing 95.4%, 70.3%, and 41.7% total excellent response, respectively. Patients with poliosis showed 54.9% in total excellent response rate, much lower than 84.5% in patients without poliosis(P<0.001). No significant response differences in gender were found.
@*CONCLUSION@#308 nm excimer laser is effective and safe in treatment of vitiligo. Aging, disease duration, lesion location, and hair color in lesion may be the influential factors for 308 nm excimer laser in treatment of vitiligo patients.

Effect of timing XiaoZhang acupoint application on nausea and vomiting after laparoscopic myomectomy surgery / 中国内镜杂志

Objective To evaluate the effect of timing XiaoZhang acupoint application deposited on nausea and vomiting after laparoscopic myomectomy surgery (Post-Operative Nausea and Vomiting, PONV). Methods 120 cases of uterine fibroids were randomly divided into experimental group and control group. There were 5 cases in the experimental group and 4 cases in the control group have lost to follow-up, and 111 cases were included in the study. The control group (n =56) accept vomiting routine nursing and selective false acupoint application therapy intervention, the experimental group (n =55) accept vomiting routine nursing and selective XiaoZhang acupoint application therapy intervention. The incidence of nausea and vomiting in two groups were observed after operation in 0, 6, 12, 24 h respectively. Results Before intervention(postoperative 0 h), intervention (postoperative 6 h), the patients in experimental group of PONV symptoms (0~ Ⅳ ) compared the control group, the difference was not statistically significant (P >0.05);Intervention (postoperative 12 h and 24 h) of the patients in the experimental group PONV symptoms (0~ Ⅳ ) and the control group, the difference was statistically significant (P <0.05). Conclusions Selective ChenShi implementation of XiaoZhang acupoint application can effectively improve laparoscopic myomectomy surgery (0 ~Ⅳ ) of PONV symptoms, and the side effect is slight.

Establishment and optimization of loop-mediated isothermal amplification for rapid detection of pseu-domonas aeruginosa / 实用医学杂志

Objective To establish a Loop-mediated Isothermal Amplification (LAMP) for Pseudomonas aeruginosa rapid detection. Method 152 P. aeruginosa strains isolated from nasal swabs and 30 reference strains were applied. P. aeruginosa ATCC15442 was used to develop LAMP amplification and evaluate sensitivity and specificity. Results Sensitivity of LAMP was 103 times higher than PCR, with DNA amount as 132 fg. When LAMP was applied to 30 reference strains and 152 P. aeruginosa strains , the specification was 100% while iden-tification rate reached 94.7%. Conclusion The establishment LAMP showed a promising prospect in P. aerugi-nosa rapid detection.

Combined ozone hydrotherapy for atopic dermatitis:evaluation of efficacy and detection of interleukin-4 and nerve growth factor levels in peripheral blood from patients before and after treatment / 中华皮肤科杂志

Objective To evaluate the clinical efficacy and safety of combined ozone hydrotherapy for the treatment of atopic dermatitis(AD). Methods A total of 60 patients with moderate or severe AD aged from 6 to 65 years were enrolled, and randomly and equally divided into a test group and a control group. Both the two groups were treated with oral levocetirizine capsules 5 mg once a day, topical tacrolimus ointment twice a day, and topical moisturizers. The test group was additionally treated with ozone hydrotherapy 3- 5 times every week. The treatment lasted 2 weeks. The severity scoring of atopic dermatitis (SCORAD) score, visual analog scale (VAS) score, dermatology life quality index (DLQI) or children′s dermatology life quality index (CDLQI) score were assessed before and after the treatment, and compared between the two groups. Enzyme?linked immunosorbent assay(ELISA) was performed to measure the levels of interleukin?4(IL?4)and nerve growth factor(NGF)in peripheral blood from the patients before and after the treatment. Results After 2?week treatment, the SCORAD scores, VAS scores and DLQI/CDLQI scores significantly decreased from 42.13 ± 16.03, 7.14 ± 2.12 and 14.92 ± 5.94 before the treatment to 27.3 ± 11.01, 2.23 ± 1.31 and 9.69 ± 4.17 respectively in the test group(all P0.05). Conclusion Combined ozone hydrotherapy can effectively and safely improve the condition of patients with AD, likely by decreasing the levels of IL?4 in peripheral blood.

Sexual dysfunction in young male patients w ith stroke / 国际脑血管病杂志

As a key aspect of quality of life in patients w ith stroke, the clinical research of sexual function and sexual satisfaction is stil rare. The rehabilitation goals in patients w ith stroke usualy include recovering functional activities and improving quality of life, how ever, it is not yet know n about the recovery of sexual function. This article review s the epidemiology, etiology, risk factors, clinical manifestations, treatment and rehabilitation of sexual dysfunction in male patients w ith stroke.

Clinic significance of neutrophil-iymphocyte ratio in the early-stage diabetic nephropathy / 天津医药

Objective To investigate neutrophil lymphocyte ratio (NLR) in early-stage diabetic nephropathy and its clinic significance. Methods The 145 subjects were divided into two groups:the healthy control group (n=54) and early stage diabetic nephropathy group (n=91). The numbers of neutrophils (N) and lymphocytes (L) as well as the NLR values of peripheral blood and other biochemistry index were examined. Factors of early stage diabetic nephropathy were calculated us?ing variance and logistic regression analysis. Results Creatinine(Cr), total cholesterol(TC), triglyceride(TG), LDL-C, neu?trophils number and CRP in DN group were significantly higher than those of the control group and lymphocytes numbers of DN group were significantly lower than that of the control group (P<0.05 respectively);NLR values were significantly higher in diabetic nephropathy group compared with those of healthy control group(2.52±0.57 vs 1.82±0.60,t=6.997, P<0.01). Lo?gistic regression analysis showed that the risk factors of DN include NLR, TG and total cholesterol. NLR ( P <0.001, OR=8.951, OR 95%CI:3.595-22.287) was significantly associated with DN. Conclusion High NLR values may be a predic?tive and reliable marker ofearly-stage DN.